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31.
本文采用短乳杆菌、酵母菌、枯草芽孢杆菌和地表芽孢杆菌进行豆粕固态限定发酵和强化发酵的研究,对发酵过程中小分子蛋白含量、菌量、pH及胰蛋白酶抑制剂活性等进行了测定.结果表明:纯菌条件下,接种短乳杆菌和地衣芽孢杆菌实验组限定发酵的小分子蛋白含量分别为31.0%和28.3%,高于接种酵母菌试验组的22.4%.且小分子蛋白积累高峰时间为48~72 h;接种地表芽孢杆菌实验组的胰蛋白酶抑制剂降解率高于接种短乳杆菌或酵母菌实验组.豆粕天然发酵试验组的小分子蛋白含量和胰蛋白酶抑制剂降解率分别为22.2%和44.1%.接种短乳杆菌、地衣芽孢杆菌试验组的小分子蛋白含量和胰蛋白酶抑制剂降解率分别为32.4%、99.5%和29.3%、99.7%.表明接种强化发酵有助于小分子蛋白含量的提高和胰蛋白酶抑制剂的降解. 相似文献
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TANG Fang . College of Forestry Resources Environment Nanjing Forestry University Nanjing China . Jiangsu Key Laboratory for Prevention Management of Invasive Specie Nanjing . ZHANG Xiu-bo LIU Yu-sheng College of Plant Protection Sh ong Agriculture University Tai’an China GAO Xi-wu Department of Entomology China Agricultural University Beijing China 《林业研究》2011,22(3):447-451
Micromelalopha troglodyta (Graeser) is an important pest of poplar in China. Glutathione S-transferases (GSTs) are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors. 相似文献
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对备受瞩目的1-MCP、乙醇和乙醛、NO和N2O等气体物质对园艺产品采后保鲜中的影响及其作用机理、特点及应用前景进行了概述. 相似文献
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为进一步研究家蚕胰凝乳蛋白酶抑制剂基因SCI-SB的功能和作用机理,利用RT-PCR技术,从家蚕总RNA中扩增到家蚕SCI-SB基因片段,构建了含有GST标签的融合表达质粒pGEX4T-1-SCI-SB。采用pGEX融合蛋白表达系统,在大肠杆菌BL21中得到以包涵体形式存在的重组GST融合蛋白质,表达量可占菌体蛋白的15%。将包涵体变性、复性处理以后,进一步利用GSTTrapFF亲合层析一步获得了重组蛋白。将该重组蛋白免疫新西兰纯种大白兔,获得效价高的多克隆抗体。胰凝乳蛋白酶活性抑制实验结果表明该重组蛋白具有一定的酶活抑制作用。 相似文献
38.
木质纤维素乙醇发酵研究中的关键点及解决方案 总被引:15,自引:6,他引:15
该文综述了近年来木质纤维素材料发酵生产乙醇的研究进展,提出了在木质纤维素原料发酵生产乙醇过程中的两大关键点,一是减少和消除原料预处理过程中抑制物及其有害影响;二是含木糖、葡萄糖、甘露糖、半乳糖等多种物质的混合物同时作为底物的乙醇发酵。在对已有研究结果比较和分析基础上,提出相应的解决方案。首先要采取综合的预处理过程,达到提高木质纤维素水解率的同时减少发酵抑制物;然后是提高发酵菌种对混合糖底物的利用能力和发酵生成乙醇的能力以及对抑制物的耐受性,以提高木质纤维素发酵生产乙醇的转化率。 相似文献
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AIM:To observe the effect of intravitreal injection of pirenzepine on form-deprivation myopia in chicks. METHODS:Forty-one day old chicks were randomly divided into 4 groups:normal control group, form deprivation group, vehicle control group and pirenzepine injection group. The right eyes of all chicks were used as experimental eyes. The deprived eyes in vehicle control group and pirenzepine injection group received daily intravitreal injection of vehicle control solution(0.01 mol/L PBS) and pirenzepine(1%in PBS), respectively. Optical examinations such as refraction, axial length and equatorial diameter were made at the end of the 5th day. The mRNA and protein levels of matrix metalloproteinase 2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2), and the activity of MMP-2 were detected by RT-PCR, Western blotting and zymography analysis,respectively. RESULTS:Refraction, axial length and equatorial diameter of the eyes in pirenzepine injection group were significantly lower than those in form deprivation group and vehicle control group(P<0.05), but those were higher(P<0.05) and the eyes were relatively myopic as compared with normal control group. The mRNA expression, protein le vels and activity of MMP-2 in pirenzepine group were significantly higher than those in normal control group(P<0.01), and were significantly lower than those in form deprivation group and vehicle control group(P<0.01, P<0.01). The mRNA and protein levels of TIMP-2 in pirenzepine group were significantly lower than those in normal control group(P<0.01), and was significantly higher than those in form deprivation group and vehicle control group(P<0.01, P<0.01). CONCLUSION:Intravitreal injection of pirenzepine may partly prevent form-deprivation myopia by modulating the expression of MMP-2 and TIMP-2 in the fibrous layer of sclera. 相似文献
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Nonsteroidal anti-inflammatory drugs (NSAIDs) are powerful in anti-inflammatory, analgesic and antirheumatic effects, and widely used in treating the corresponding diseases. Over the years, many new dosage forms and structures of NSAIDs appear since aspirin was developed 112 years ago. However, the universal use of NSAIDs produces unavoidable mucosal lesions in gastrointestinal tract. As yet, proton pump inhibitor (PPI) has been used in the treatment of gastropathy induced by NSAIDs. This article will focus on the advances in prevention of NSAIDs-induced gastropathy by proton pump inhibitor. 相似文献